Regulatory
Part:BBa_K632003:Design
Designed by: Lauren Cole Group: iGEM11_Minnesota (2011-09-28)
Ho1-PcyA-cph8, regulatory system
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2257
Illegal BglII site found at 3038
Illegal XhoI site found at 364 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2591
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Ho1 and pcyA were put under the same inducible promoter, and cph8 was put under a separate inducible promoter.
Source
Heme oxigenase (ho1) was obtained from a pMOD plasmid library vector. Phycocyanobilin:ferredoxin oxidoreductase (pcyA) was obtained from genomic DNA from Synechocystis sp. PCC 6803. Cph8 from BioBrick: BBa_I15010.